Full time, fixed-term appointment to 31<sup>st</sup> December 2026 Based in Liverpool with international tr.....
Full time, fixed-term appointment to 31<sup>st</sup> December 2026
Based in Liverpool with international travel
We have an exciting opportunity for a Post-Doctoral Research Associate to join the Vector Biology team at LSTM. Primarily the successful candidate will be required to support developing functional genetics tools for the study of insecticide resistance in Anopheles funestus.
Background
Anopheles funestus mosquitoes are the dominant vectors of malaria in many regions of sub-Saharan Africa, yet this species has hitherto been understudied due, in part, to the difficulty in rearing strains in the laboratory and a lack of functional genetics tools to discover the genetic basis of key biological traits. Nonetheless, our research, using genomic surveillance, shows that insecticide resistance is compromising malaria control efforts that look to suppress this mosquito and that the genetic basis of these resistance mechanisms is often unique from that emerging in other malaria-transmitting species.
Given the paucity of novel classes of insecticides, resistance management strategies such as rotation, mosaics, mixtures and combinations are essential to prolong the susceptibility of mosquito vectors to insecticides. This cannot be properly done without understanding the molecular drivers of insecticide resistance.
The groups of Wondji and Nolan are world leading in the discovery of insecticide resistance mechanisms and the development of functional genetics tools, respectively. They have recently developed efficient genome editing technologies for Anopheles funestus, allowing the precise introduction of transgenes and/or precise genome ‘edits’ that faithfully recapitulate alleles that appear to be under selection in the wild. These tools, for the first time, allow us to functionally validate insecticide-resistant alleles, and potentially novel resistance pathways, in the laboratory.
The candidate
The successful candidate will have significant experience in molecular biology and cloning of genetic constructs. You will demonstrate excellent research skills, analytic expertise, and communication skills, plus the ability to work as a member of a multi-disciplinary team.
As the Post-Doctoral Research Associate, you will be involved in liaising with field scientists and genomics specialists to prioritise mutations or genomic rearrangements that appear to be under selection in response to various insecticide deployments. The post will involve working in collaboration with and travel to LSTM research collaborators abroad, particularly with researchers from the Centre for Research on Infectious Diseases in Cameroon.
Key responsibilities are (but not limited to):
Develop and expand genome editing approaches in mosquitoes: Design and clone new genetic constructs for optimized performance in the mosquito; Use CRISPR technologies to edit mosquito genomes; Optimise a screening protocol to recover genome-edited mosquitoes without the use of a dominant selectable marker.
Develop and expand systems for selecting for insecticide resistance in vivo: To learn the basic standardized insecticide resistance testing assays and to perform these with sufficient statistical power; Establish a screening protocol and selection regime for emergence of insecticide resistance in mosquitoes; Liaise with field scientists and genomics specialists
Provide support and supervision to PhD students and technical staff: Create training material for, and provide training in molecular and analytical techniques; Assist in the design and supervision of student projects; Assist in training and mentoring staff;
Develop novel projects: Generate ideas and data to support new project applications; Contribute to the writing of research grants/ fellowships in collaboration with PIs; Disseminate findings through publications and conferences.
The ideal candidate will demonstrate:
PhD in Molecular Biology, Genetics, Biochemistry or mosquito biology/entomology
Significant experience in molecular biology and cloning of genetic constructs
Experience or knowledge of genome editing approaches using site-specific nucleases such as CRISPR, TALENs, Zinc Finger Nucleases etc
Basic bioinformatic skills (e.g. genome browsing and data mining, use of DNA cloning software packages
Evidence of preparing publications for specialist/ general science journals
Experience working with mosquitoes or other insects (desirable)
(Please refer to the job description and person specification for the full list of essential and desirable criteria)
Informal enquiries can be made to: tony.nolan@lstmed.ac.uk
Additional benefits of joining LSTM:
30 days annual leave, plus bank holidays, plus Christmas closure days
Generous occupational pension schemes
Government backed “cycle to work” scheme.
Affiliated, discounted staff membership to the University of Liverpool Sports Centre
Closing Date: 3<sup>rd</sup> January 2024
Interviews are expected to take place mid- January
Application Process: To apply for the position please follow the apply link and upload your CV and covering letter.
Due to the volume of applications, we receive, we may close our vacancies early. It is therefore advisable to apply as early as possible if you would like to be considered for a role.
Inclusion is central to our values at LSTM.
We seek to attract and recruit people who reflect the diversity across our communities, regardless of sexual orientation, gender identity, ethnicity, nationality, faith or belief, social background, age and disability. LSTM selects candidates based on skills, qualifications, and experience.
We welcome conversations about flexible working; and applications from those returning to employment after a break from their careers.
About LSTM
Founded in 1898 and the oldest of its kind in the world, the Liverpool School of Tropical Medicine (LSTM) is an internationally recognised centre of excellence for teaching and research in tropical diseases. Through the creation of effective links with governments, NGOs, private organisations and global institutions and by responding to the health needs of communities, LSTM aims to promote improved health, particularly for disadvantaged populations.
Look at some of the great work we have achieved over the past year by viewing our annual report:
LSTM Annual Report 2021 - 2022
LSTM actively promotes Equal Opportunities and Safeguarding Policies
Please note the Recruitment Office will be closed from 20<sup>th</sup> December, and re-opens on 2<sup>nd</sup> January 2024.